GEN220_2023

2023 Class

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Range queries

Genomic arithmetic with BedTools - https://bedtools.readthedocs.io/en/latest/

Often want to ask questions about genomic ranges. For example.

• What are genes that are found on Chromosome 1?
• What are sequence reads that are aligned to Gene X
• How many RNA sequence reads are aligned to Protein coding genes?

Some starter example code:

https://github.com/biodataprog/GEN220_2019_examples/tree/master/Bioinformatics_1/Ranges

#!/usr/bin/bash
module load bedtools

bedtools intersect -a rice_chr6.fixed_Chr.gff -b rice_chr6_3kSNPs_filt.bed -wo > snp_gene_intersect.tab

# how many features have SNPS?
cut -f3 snp_gene_intersect.tab | sort | uniq -c

# how many SNPs does each gene have?

grep -P "\tgene\t"  snp_gene_intersect.tab > snp_gene_intersect.genes_only.tab
# this outputs gene SNP counts ordered by genename which is actually chromosome
# position nicely
cut -f9 snp_gene_intersect.genes_only.tab | sed 's/^ID=//; s/;Name=.*//' | sort | uniq -c > gene_snp_count.txt

# which genes have the most snps?
sort -nr gene_snp_count.txt > gene_snps_count.by_number.txt